DMEM/Agarose cloning mixture (for base and top layers): mix the following and keep at 37 C: 2 x DMEM 25.0 ml, BSP 10.0 ml, 50x HAT 1.2 ml, 1.2% agarose 25.0 ml

Cloning of Animal Cells
... Use semi-solid culture medium. Often contains low concentration of agar or agarose; Base layer of agar medium is placed into a petri dish; ...
www.bmb.psu.edu/courses/biotc489/notes/cloning.htm

Quantitating a Mutagen
... soft agar overlay until it completely covers the surface of the minimal agar base layer. ... What would you see on your petri dish of mutant bacteria on DMA and why ...
www.bio.davidson.edu/Courses/Bio111/ Bio111LabMan/Lab%209.html